College of Medicine and Dentistry
|AVAILABILITY||10am -4pm by appointment|
|PRIMARY CONTACT||Roger Bird|
|SECONDARY CONTACT||Dale Taylor|
|ORGANISATION||University of Birmingham|
The Legacy is equipped with three lasers and can detect up to 11 parameters. It is routinely used for high speed sorting with up to 50,000 cells per second, single cell sorting into PCR plates for gene expression analysis or cloning.
Capabilities- 1 Sort 4 selected populations simultaneously 2 Enrich cell populations at rates of up to 80000 cells/sec 3 Puritysorts at rates of up to 30000 cells/sec 4 Sort cells directly into multi-well plates
Under the right conditions purity levels of >99 per cent and recoveries of >95 per cent can be achieved
lasers- 360,488,and 633nm 11 analysis channels- Forward scatter, side scatter, 5 fluorescence channels activated by 488nm laser 2 fluorescence channels activated by 360nm laser 2 fluorescence channels activated by 633nm laser
Suitable fluorophoresinclude FITC, RPE, PE-Cy5, PE-Cy7, PE-Texas Red, Hoechst, APC and APC-Cy7
Last Updated: 24th April, 2013
- Flow Cytometry
This M5 equipment data is licensed under the UK Open Government Licence.