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Confocal Laser Scanning Microscopes

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MANUFACTURER Leica Microsystems
MODEL TCS SP5 II

Life Health Sciences

RESTRICTIONS Health and safety procedures must be followed.
TRAINING Training is required to use this item and we can arrange this if needed.
PRIMARY CONTACT astonkit@aston.ac.uk
ORGANISATION Aston University

Description

True confocal scanning microscopy uses a pinpoint light source in the form of a laser to illuminate samples, a small section at a time. A scanner then drives the laser over the sample, generating an image. Exciting samples in this way produces less out of focus light than wide-field microscopy giving higher resolution images. We have a variety of objectives and 8 visible laser lines, allowing for a wide range of samples to be imaged. Confocal microscopy gives high resolution 3D images and sequences; This system has a multiphoton laser which is capable of penetrating up to 700um deep, enabling us to look deep into tissue samples. The resonant scanner allows us to capture high speed events such as waves of molecules moving through cells.

Specification

10x and 20x dry objectives

10x and 20x water objectives

40x and 63x oil objectives

Upright Microscope

High resolution Scanner

Fast resonant scanner 8 Visible Laser Lines Tunable Multiphoton Laser z -stacking

Time Lapse Sequential Imaging

FRAP and FRET Wizard

Analysis Computer Training and support provided Online booking system

Last Updated: 8th January, 2014

Confocal Laser Scanning Microscopes Aston University

This M5 equipment data is licensed under the UK Open Government Licence.